- The tasks below are examples to practice data tidying and cleaning in excel and generating figures from example data
Suggested prerequisite(s)
- The Data tidying and figure generation tutorial
- An understanding of tidy data. See https://www.youtube.com/watch?v=KW1laBLEiw0
Dataset
This task uses the DataHandlingAndPresentation.xlsx dataset
Task 1: Data tidying and cleaning (Table sheet in dataset)
You have done an experiment where you determined the following things:
- The minimal inhibition concentration (MIC) of a drug
- Tested range: 1-64ug/ml
- The presence or absence of a plasmid using PCR
- The presence of a mutation in a specific gene using PCR
However, the data is not in a tidy format (samples in rows, measurements in columns, 1 entry per cell) and the data has not been cleaned of errors.
You should:
- Tidy the data into a single table using the data tidying principles outlined previously
- Remove any obvious errors from the data (i.e. clean the data)
- Create a proper caption for this table and an informative legend
You can find a sample answer sheet here.
Task 2: Choose and create appropriate figures for different data (Figure 1 and Figure 2 sheets in dataset)
You have done 2 experiments:
- Figure 1: A comparison study to see whether an improvement in S. epidermidis morbidity figures occurs when devices infused with different substances are used compared with when traditional devices are used
- Figure 2: Looking at the relationship between MIC and qPCR CT values for a resistance-associated gene in E. coli
You should:
- Create 2 figures, one per study (see links below for guides)
- Select the correct chart type for each set of results
- Create the charts in excel with informative axes labels and accessible colour schemes
- Insert a linear regression line, if appropriate (e.g. scatterplot)
- Create a proper caption for each figure with informative legends
You can find a sample answer sheet here.
Online guides for making charts in excel
Scatterplot
https://www.youtube.com/watch?v=gBbGBrHTMrM&ab_channel=Vertex42
https://www.youtube.com/watch?v=kLROcLFzH8o&ab_channel=BurkeyAcademy
Linear regression and scatterplot
https://www.youtube.com/watch?v=L_a8Z0BVjyM&ab_channel=MonaSchraer
https://www.youtube.com/watch?v=Cltt47Ah3Q4&ab_channel=JalayerAcademy (detailed)
[https://www.youtube.com/watch?v=9wX1a1J4WOI&ab_channel=TopTipBio(https://www.youtube.com/watch?v=9wX1a1J4WOI&ab_channel=TopTipBio)] (very detailed, with analysis pack)
[https://toptipbio.com/linear-regression-excel/(https://toptipbio.com/linear-regression-excel/)] (step by step guide with last video)
Bar chart
https://www.youtube.com/watch?v=U5MY9m9mG2c&ab_channel=TopTipBio
Bar charts using Excel worksheet
Box and whisker
https://www.youtube.com/watch?v=39lsUsJsc2c&ab_channel=TopTipBio
Box plots using Excel worksheet